{"title":"Notch","description":"\u003cp\u003e\u003cspan style=\"font-size: 11.0pt; line-height: 115%; font-family: 'Georgia',serif; mso-fareast-font-family: 'MS Mincho'; mso-fareast-theme-font: minor-fareast; mso-bidi-font-family: Arial; color: black; mso-color-alt: windowtext; background: white; mso-ansi-language: EN-US; mso-fareast-language: JA; mso-bidi-language: AR-SA;\"\u003eNotch1 is a cell surface protein that has diverse roles in human biology at all stages of life. Notch1 is modified by a number of sugar structures, including a number that are termed \u003ci style=\"mso-bidi-font-style: normal;\"\u003eO-\u003c\/i\u003efucose. Alteration of the pattern of \u003ci style=\"mso-bidi-font-style: normal;\"\u003eO-\u003c\/i\u003efucose structures found on Notch1 has significant effects on Notch1 function, which in turn has been linked to both developmental and adult pathologies, including cancer.  GlycoScientific has developed a collection of antibodies against fucose modified \u003cspan\u003eEpidermal growth factor-like repeats on Notch1 to aid in the study of this integral protein.  \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e","products":[{"product_id":"anti-notch-1-egf8-polyclonal-antibody","title":"Anti-Notch1 EGF8 (O-Fuc Thr311) Polyclonal Antibody","description":"\u003cp\u003e\u003cstrong\u003eDescription    \u003c\/strong\u003eRabbit polyclonal antibody to human epidermal growth factor-like repeat 8 (EGF-8) modified at threonine 311 (Thr311) with an O-linked fucose (O-Fuc). GS0064 preferentially binds to the O-fucosylated version of EGF8. Amino acid sequence of the region flanking Thr311 suggests cross-reactivity with EGF of multiple species including mouse, rat and zebra fish.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTested applications\u003c\/strong\u003e  Direct ELISA\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget sequence\u003c\/strong\u003e       QNGGT(O-Fuc)CHNTH\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIsotype                       \u003c\/strong\u003eMixed, predominantly IgG.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0621\/8253\/files\/EGF_8_Fuc_PAb_CofA_GS0064_A099.pdf?v=1787849174\" rel=\"noopener\" target=\"_blank\"\u003eClick here for a sample data sheet.\u003c\/a\u003e\u003c\/p\u003e","brand":"GlycoScientific","offers":[{"title":"0.1mL","offer_id":46489130434657,"sku":"GS0064-100","price":400.0,"currency_code":"USD","in_stock":true},{"title":"0.025mL","offer_id":46489130467425,"sku":"GS0064-25","price":140.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0621\/8253\/products\/2ndresizedistockantibody_b4012b31-72a7-48f0-b493-56b6401975a1.png?v=1660075244"},{"product_id":"anti-notch1-egf12-to-fuc466-monoclonal-antibody","title":"Anti-Notch1 EGF12 (O-Fuc Thr466) Monoclonal Antibody","description":"\u003cp\u003e\u003cstrong\u003eDescription    \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\" style=\"text-align: left;\"\u003eRabbit monoclonal antibody to human Epidermal growth factor-like repeat 12 (EGF-12) modified at threonine 466 (Thr466) with an O-linked fucose (O-Fuc). GS0331 binds to the O-fucosylated version of EGF12, and also shows binding to other O-gucosylated growth factor-like repeats 8 (Thr311) and EGF26 (Thr997).\u003cspan style=\"mso-spacerun: yes;\"\u003e  \u003c\/span\u003eThe mAb does not detect non-fucosylated forms of these sequences.\u003cspan style=\"mso-spacerun: yes;\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\" style=\"text-align: left;\"\u003e\u003cspan style=\"mso-spacerun: yes;\"\u003eFurther validation of this monoclonal was conducted on the EGF portion of Notch1 proteins produced in E. coli.  In vitro treatment of these proteins with glycosyltransferase POFUT1, which extends O-fucose on properly folded proteins results in a terminal fucose monosaccharide.  Further addition of the Lunatic Fringe (Lfng) glycosyltransferase in the presence of UDP-GlcNAc results in an O-Fucose containing disaccharide.  The GS0331 EGF12 mAb is able to preferentially detect reduced forms of the POFUT1 treated EGF proteins.  Treatment with PNGaseF does not inhibit detection, further \u003c\/span\u003everifying that the antibody is specific to an O-linked sugar rather than n-linked glycosylation (data not shown).\u003cspan style=\"mso-spacerun: yes;\"\u003e    \u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"left\" style=\"text-align: left;\"\u003e\u003cspan style=\"mso-spacerun: yes;\"\u003ePlease inquire for availability of this mAb.  Production lead times may be associated with ordering this product.     \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTested applications\u003c\/strong\u003e  Direct peptide ELISA, Western blot\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTarget sequence\u003c\/strong\u003e      QNDAT(O-Fuc)CLDQ\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0621\/8253\/files\/EGF_12_Fuc_mAb_CofA_GS0331_A484.pdf?v=1787926087\" rel=\"noopener\" target=\"_blank\"\u003eClick here for a sample data sheet.\u003c\/a\u003e\u003c\/p\u003e","brand":"GlycoScientific","offers":[{"title":"0.1mL","offer_id":46491378647137,"sku":"GS0331-100","price":400.0,"currency_code":"USD","in_stock":true},{"title":"0.025mL","offer_id":46491378679905,"sku":"GS0331-25","price":140.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0621\/8253\/products\/2ndresizedistockantibody_b4012b31-72a7-48f0-b493-56b6401975a1.png?v=1660075244"}],"url":"https:\/\/www.glycoscientific.com\/collections\/notch.oembed","provider":"GlycoScientific","version":"1.0","type":"link"}