Publications and Presentations

 GlycoScientific is proud to provide quality reagents that are being used in cutting edge research.  Check out our literature library below to learn more about how GlycoScientific products are being used to advance discovery and knowledge among a diverse array of research topics.

Publications

Do I Need to Trypsin Digest Before Releasing IgG Glycans With PNGase-F 

Birx L, Popov M, Orlando R. J Biomol Tech. 2023 Mar 1:3fc1f5fe.6db6338a. doi: 10.7171/3fc1f5fe.6db6338a.

 

A homogeneous bioluminescent immunoassay for parallel characterization of binding between a panel of antibodies and a family of Fcγ receptors.         

Nath, N., Godat, B., Flemming, R. et al. Sci Rep 12, 12185 (2022). https://doi.org/10.1038/s41598-022-15887-z

 

MS-based glycomics: An analytical tool to assess nervous system diseases.                                                                                                           

Peng W, Kobeissy F, Mondello S, Barsa C, Mechref Y. Front Neurosci. 2022 Nov 3;16:1000179. doi: 10.3389/fnins.2022.1000179. PMID: 36408389; PMCID: PMC9671362.

 

Detection and Analysis of Proteins Modified by O-linked Acetylglucosamine.                                                                                    

Zachara NE, Vosseller K, Hart GW. Curr Protoc Mol Biol. 2011 Jul;Chapter 17:Unit 17.6. doi: 10.1002/0471142727.mb1706s95. PMID: 21732316; PMCID: PMC3329785.

 

Deciphering the Interaction between Neonatal Fc Receptor and Antibodies Using a Homogeneous Bioluminescent Immunoassay                                      

Nath N, Godat B, Flemming R, Urh M. J Immunol. 2021 Aug 15;207(4):1211-1221. doi: 10.4049/jimmunol.2100181. Epub 2021 Jul 26. PMID: 34312257; PMCID: PMC8358922.

 

Tools for Functional Dissection of Site-Specific O-GlcNAcylation.               

Gorelik A, van Aalten DMF. RSC Chem Biol. 2020 Jun 12;1(3):98-109. doi: 10.1039/d0cb00052c. PMID: 34458751; PMCID: PMC8386111.

 

Compensated Hydroxyl Radical Protein Footprinting Measures Buffer and Excipient Effects on Conformation and Aggregation in an Adalimumab Biosimilar.                                                                                                        

Misra SK, Orlando R, Weinberger SR, Sharp JS. AAPS J. 2019 Jul 11;21(5):87. doi: 10.1208/s12248-019-0358-2. PMID: 31297623; PMCID: PMC7670911.

 

Inline Liquid Chromatography-Fast Photochemical Oxidation of Proteins Allows for Targeted Structural Analysis of Conformationally Heterogeneous Mixtures.                                                                                                          

Tadi S, Misra SK, Sharp JS. Anal Chem. 2021 Feb 23;93(7):3510-3516. doi: 10.1021/acs.analchem.0c04872. Epub 2021 Feb 9. PMID: 33560821; PMCID: PMC7951798.

 

Predicting the HILIC Retention Behavior of the N-Linked Glycopeptides Produced by Trypsin Digestion of Immunoglobulin Gs (IgGs).              

Badgett MJ, Mize E, Fletcher T, Boyes B, Orlando R. J Biomol Tech. 2018 Dec;29(4):98-104. doi: 10.7171/jbt.18-2904-002. PMID: 30479582; PMCID: PMC6237148.

 

Enhanced Quantitative LC-MS/MS Analysis of N-linked Glycans Derived from Glycoproteins Using Sodium Deoxycholate Detergent.                       

 Zhu R, Zhou S, Peng W, Huang Y, Mirzaei P, Donohoo K, Mechref Y. J Proteome Res. 2018 Aug 3;17(8):2668-2678. doi: 10.1021/acs.jproteome.8b00127. Epub 2018 Jul 20. PMID: 29745666.

 

Reliable LC-MS quantitative glycomics using iGlycoMab^TM stable isotope labeled glycans as internal standards.                                                         

Zhou S, Tello N, Harvey A, Boyes B, Orlando R, Mechref Y. Electrophoresis. 2016 Jun;37(11):1489-97. doi: 10.1002/elps.201600013. Epub 2016 Apr 9. PMID: 26913967; PMCID: PMC4964797.

Presentations

September 2018 Profiling the N-Glycosylation of Biotherapeutic IgGs with HILIC-MRM Analysis of Trypsin Digested Culture Media (CASSS 2018)

June 2018  Absolute Quantitation of N-linked Glycans attached to Biotherapeutics with Isotopically Labeled Internal Standards (ASMS 2018)

June 2017 Profiling the N-Glycosylation of Biotherapeutic IgGs with HILIC-MRM Analysis of Trypsin Digested Culture Media (ASMS 2017)

May 2017   GlycoScientific Developments and characterizes of antibodies to site-specific O-GlcNAc modified histones for epigenetic research (ABRF 2017).

 

March 2015   iGlycoMab^TM an outstanding scientific presentation award at the 2015 conference of the Association of Biomolecular Resource Facilities  (ABRF 2015).  Click here to see a copy of the award wining poster.

May 2015   GlycoScientific Raises the Accuracy and Precision Standard for Glycan Quantitation with the release of iGlycoMab^TM .  GlycoScientific introduces a stable isotope labeled monoclonal antibody (mAb) (iGlycoMab^TM ) designed to facilitate the accurate and precise quantitative glycan profiling of biological therapeutics, particularly monoclonal antibodies and fusion proteins. However, iGlycoMab^TM can be applied to individual glycans or complex mixtures such as those observed in the field of glycomics.  

May 2015   iGlycoMAb^TM was highlighted in various presentations at the 2015 American Society for Mass Spectrometry (ASMS) conference.  Watch Dr. Ron Orlando's oral presentation on the benefits of iGlycoMab^TM .